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These are usually derived from viral genes, which explain their high immunoreactivity

248 In clinical trials, Blanc and colleagues 249 treated a female fetus diagnosed with severe OI type III and received prenatal transplantation with 6.5 10 6 cells/kg allogeneic HLA-mismatched male fetal MSCs at 32 weeks of gestation

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Gene expression levels were determined by qRT-PCR in the Quantifast SYBR Green PCR mix (Qiagen GmbH, Germany) with a reaction condition of initial denaturation at 95 C for 5 min and then 40 cycles of 95 C for 10 s and 60 C for 30 s in QuantStudio 3 machine