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Intramuscular (IM) injections of hydroxocobalamin are the most reliable method of ensuring adequate B12 levels, particularly in patients with confirmed deficiency or neurological symptoms
The creatine supplementation also promoted energy reserve in the muscle of spotted seabass by increasing ATP concentrations and creatine kinase activity, while inhibiting anaerobic glycolysis by reducing the activities of lactate dehydrogenase and pyruvate kinase [38]
2.9 Cell culture and treatment Mouse colon epithelial cells (MCECs) were cultured in high-glucose Dulbeccos Modified Eagle Medium, supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (P/S), in a humidified incubator with 5% CO 2 at 37C
