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Western Blot Analysis Cellular and cerebral samples were lysed in RIPA (Beyotime, China) containing inhibitor cocktail (Roche, Germany) for 20 min, following which the lysates were centrifuged (12,000 g) at 4C for 15 min
Continuous administration of any bioactive compound can lead to downregulation of its target receptors, diminishing its effects over time
An example is a 2020 meta-analysis examining the effect of KD on epilepsy treatment [207]
YWHAZ contributes to chemotherapy resistance and recurrence of breast cancer (Ralhan et al., 2008)
