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Unlike previous preconditioning ischemia/reperfusion studies (Danduga et al., 2018

Laboratory protocols demonstrate that under optimal dosing conditions, the peptide eliminates senescent cells while destroying an undetectable number of healthy cells, representing exceptional selectivity compared to earlier senolytic approaches

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Relative mRNA expression was determined using the GoTaq 1-step real-time quantitative PCR (RT-qPCR) system (TAKARA) and qPCR using SYBR premix Ex Taq kit (TAKARA), and RT-qPCR was performed on an ABI Prism 7500 Fast Real-Time PCR System (Applied Biosystems, CA, USA)