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Tissues were homogenized in 510 mL of cold buffer (50 mM potassium phosphate, pH 7.5, 1 mM EDTA) per gram of tissue, followed by centrifugation at 10,000 g for 15 min at 4 C
4K), also interferes with PI3K signaling [58]
Jessica Drummond (00:35:45) So 19
