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There are mechanisms that also promote extrusion of Ca 2+ from the intracellular space to the extracellular space via membrane pumps such as the plasma membrane Ca 2+ -ATPase (PMCA), and the Na + /Ca 2+ exchanger which is activated by elevated levels of Ca 2+ and is dependent on the Na + gradient, regulated by the membrane Na + /K + ATPase

All authors were involved in the manuscript preparation, and have read and approved the manuscript

Abstract Immobilization of small proteins designed to perform proteinprotein assays can be a difficult task

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