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Description
In brief, the sheaths and stems of 2-week-old rice seedlings were cut into 0.5-mm strips and soaked in cell-wall digestion buffer (1.5% cellulase RS, 0.75% macerozyme R-10, 0.6 M mannitol, 10 mM MES, pH 5.7, 10 mM CaCl 2 , 0.1% BSA) for 6 h at 25 C in the dark with gentle shaking (4560 rpm)

The measurement of EGR activity is widely used to determine riboflavin status

Interestingly, transgenic animals with partial loss of imprinting of this locus show significant lethality in the third postnatal week, associated with developmental delay and failure to maintain UCP1 expression in BAT (141)

Weaving, R
