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The tubular protective effects appeared to be associated with the counteraction of PPAR deterioration, resulting in a maintenance of FAO, a decrease of intracellular accumulation of undigested FFAs, and attenuation of disease developmental factors including oxidative stress, apoptosis, and NFB activation (Figure 2)

In the substrate oxidation stress test, the cells were prepared as above prior to the assay, and the cells were subjected to (in sequence, in final concentrations): (1) 0, 4 mM l-glutamine (Seahorse Bioscience, Billerica, MA, USA), 40 M Etomoxir (11969, Cayman Chemical, Ann Arbor, USA) or 3 M BPTES ((bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide)

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Energy-Production Pathway Studies B12 contributes to experimental models involving ATP production, mitochondrial function, and metabolic activity