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I mean, if you look at formal exercise sessions in a gym or health club, that's something that would have been relegated to the realm of the extreme bodybuilder or the, you know, the gladiator, the warrior, the athlete, the folks for whom performance is a pretty important metric

For human fibroblasts, cells were fixed with methanol/acetone (1:1) for 15 min, permeabilized in 0.1% (v/v) Triton X-100 for 15 min and incubated with primary antibodies overnight at 4 C

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