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Add 50 L of 1 SDS-PAGE loading buffer to each tube and boil for 5 minutes

A cGAS-mediated mechanism in naked mole-rats potentiates DNA repair and delays aging

Nuclear proteins are extracted from the pellet containing pure nuclear fraction, with the help of nuclear extraction buffer, which also has proteasome inhibitors to prevent nuclear protein degradation (Sundar and Tamizhselvi, 2020)
FJC staining revealed extensive neuronal degeneration in peri-hematomal regions post-ICH
