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The cell suspension was centrifuged at 500 g for 5 min at 4C and the cell pellet was incubated with ACK lysing buffer (Thermo Fisher Scientific, A1049201) for 5 minutes to lyse the red cells

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Therefore, the efficiency of capturing and elution of 10 g APC was determined using both MyOne and M-270 beads

The depletion of PYURF leads to K562 cell death in galactose media and reduces oxygen consumption, indicating its crucial role in the generation of ATP by the oxidative phosphorylation process ( FIGURE 9 8 On the importance of Trm112/TRMT112 proteins for their MTase partners Since the identification of TRMT112 proteins as intrinsic subunits of several MTase holoenzymes, numerous biochemical and structural studies have provided a wealth of information allowing a better understanding of the roles of TRMT112 proteins on these MTases