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Dispersal and the metapopulation paradigm in amphibian ecology and conservation: Are all amphibian populations metapopulations
The absorbance at 570 nm was measured using a microplate reader (Epoch, BioTek, US)
The advantages of this sensitive technique are that the bimane derivatives have a high fluorescence yield and are quite stable (up to several days in solutions kept in the dark), and that other thiols present in the extract, such as cysteine, can be separated and measured in the same run with glutathione (Figure 2A, top)
a PDB ligand codes: GSH, Glutathione
