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Description
Correlation between diffusion in lumbar intervertebral disks and lumbar artery status: evaluation with fresh blood imaging technique

Three or four cycles between room temperature and refrigerator cause cumulative damage that compounds with each cycle

At least one of each treatment group were stained and imaged at the same time

Avoid repeated freeze-thaw cycles in the reconstituted form every freeze-thaw cycle damages peptide structure and reduces potency
