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After incubation, and washes with PBS, appropriate secondary fluorescent antibodies (1:500, AlexaFluor-conjugated, Invitrogen) were added to the blocking solution at room temperature for 2 h in the dark

(34) reported decreases in SOD and GPx activities in Chang liver cells stressed with 150 M t-BHP for 24-h

Different genes or different alleles of a single gene might be involved in each case analyzed
