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Description
Antioxidant enzymes and inflammatory markers were measured using enzyme-linked immunosorbent assay (ELISA), and HbA1c levels were assessed
So why are his-tags and nickel agarose beads so frequently used
In addition to being produced naturally by the body, glutathione can be given intravenously, topically, or as an inhalant
Nickel beads are blue (left), but that color can change during protein purification. If the blue color doesnt return and your column is still white after elution, this means that you stripped the nickel ions off of the agarose beads, likely by using too much EDTA in your buffers
