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If the acyl chains at the end hydroxyl groups (1 & 3) of glycerol are different, the center carbon becomes a chiral center and enantiomeric configurations must be recognized

The derivatization protocol for the quantification of TMA was an adaptation of the protocol described by Samples for TMAO levels quantification were prepared as follows: an amount of 25 L of each sample was collected, and 3 L of D9-TMAO (5 M concentration) and 122 L of a cold acetonitrile:methanol mixture (50:50, v/v) were added for protein precipitation

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