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Generally, changes in the protein sequence are introduced via site-directed mutagenesis techniques

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3.4 Quantitative analysis of lipid particle sub-types To estimate the relative abundances of the different lipid particles in Nile Red 2p-FLIM images, we modified the previous ensemble machine-learning approach for BCARS imaging (Figure 4A shows the results of intestinal lipid particle segmentation with the local maxima as the markers for watershed seeding