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We simulated the effect of pifithrin- treatment by multiplying the phospho-p53-dependent production of p21 and MDM2 with k PFT = 0.5, and the amount of phospho-p53 and the phosphorylation rate of p53 with k PFT phos = 0.67 at the time of treatment, because the effect of pifithrin- causes approximately a 50% decrease in p53-dependent transcriptional activation 95 and a 33% decrease in phosphorylated p53 species 93

For this purpose, adult sheep slaughterhouse ovaries were collected postmortem from the same abattoir used in Experiment 1, receiving sheep from surrounding areas during late autumn and winter

This synergistic effect was most pronounced with AS + RESV

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