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RT-qPCR The other portion of the liver was excised and immediately immersed in RNA-later solution (Ambion, Thermo Fisher Scientific, Waltham, MA, USA) to stabilize the RNA

During this period, Dixie Lawrence Tafoya, combining elements of both treatments with the addition of new ingredients, developed a combination of targeted nutritional intervention (TNI) supplements that included amino acids and smart drugs (Piracetam) in addition to various micronutrients

For solid medium experiments, spent L-DOPA medium was supplemented with 2% sterile agar and dispensed into a 48-well plate