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The ability to measure bioenergetics parameters in cells depends on sensitive measurement of dioxygen consumption before and after carefully defined addition of first an ATPase inhibitor (oligomycin) to define ATP production in the respiring cells, then an uncoupler [carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP)] to allow maximal respiration, then inhibitors (rotenone and antimycin A), as shown in Fig

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