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qRT-PCR was performed using pre-designed TaqMan Gene Expression Assay (ThermoFischer Scientific) with at least three samples in triplicates

Nature 585(7823):113118 Uddin MS, Kabir MT, Al Mamun A, Abdel-Daim MM, Barreto GE, Ashraf GM (2019) APOE and Alzheimers disease: evidence mounts that targeting APOE4 may combat Alzheimers pathogenesis

Follow up studies showed that, while aged APOE4 mice possess bioenergetic deficits in the hippocampus (Hip) and cortex (Ctx), the EC of these mice appears to possess unique counterbalancing mechanisms that allow it to resist these APOE4 -associated decreases in mitochondrial respiration

doi: 10.1177/1933719115612137 145 LinJWuDJiaLLiangMLiuSQinZet al