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Indications and Usage: Methotrexate is used to treat certain types of cancer or to control severe psoriasis or rheumatoid arthritis

Quantitative real-time PCR Total RNA was extracted from mice midbrain tissues using TRIzol reagent according to the protocol provided by the manufacturer (TaRaKa)

Under conditions of low copper levels, ATP7A resides in the trans-Golgi network (Nyasae et al., 2007)
