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(2) molecular dynamics simulated annealing restraining FOXO4 FH (without flexible regions) harmonically to its template structure (using a non-crystallographic force constant of 10,000 kcal mol 1 2 ), with additional dihedral angle restraints from 1 H, 15 N, and 13 C chemical shifts using TALOS-N 56 , hydrogen bond restraints (region p53 residues 48-55

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We thank Vitamin Diagnostics and Doctor's Data for providing testing for this study

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3.2.4 Intestinal changes and bone health While we do not know of any study directly linking the effect of aging on gut barrier-to-bone signaling, the intestinal changes that occur with aging are associated with bone loss in other conditions

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