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However, the results may plateau or wear off if not maintained

These bonds can be broken up when a water molecule is added and the resulting thing is a mix of shorter length amino acids, also called peptides

Samples which were subsequently pooled and labeled with sample tags (BD Bioscience, BD Mouse Single-Cell Multiplexing Set, 626545, rat anti-mouse MHC-H2 class I [clone M1/42]) according to the manufacturers protocol

These reactions depend on an adequate supply of folate, vitamin B12, vitamin B6 and other methylation cofactors