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Angiogenesis Inhibition: Angiogenesis, the formation of new blood vessels, plays a critical role in tumor growth and metastasis

As a further step toward the replication of thick complex tissues, such as the cardiac one, more recently 3D bioprinting has been established as a cutting-edge technology to print not only biomaterials, but also multiple cell types, with unparalleled control over their positioning, hence achieving the overall replication of the structure and composition of the native tissue

After initial growth to an OD600 of 0.8, cells were induced using 0.4 mM isopropyl -D-1-thiogalactopyranoside (IPTG) and cultured at 20C for 16 hours

Last, A., Maurer, M., Mosig, A