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All family members from both families were then screened for confirmation for the presence of the identified mutation in the MLH1 gene by Sanger sequencing using standard protocols on an ABI 3730xl instrument using the following oligomers purchased from PxLence (Ghent, Belgium) (Cat #: PXL A0182967

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H., Inhalable mRNA Nanoparticle With Enhanced Nebulization Stability and Pulmonary Microenvironment Infiltration, ACS Nano 18 (2024): 2420424218

In vitro ADME studies In vitro ADME studies comprised the assessment of plasma stability, metabolic stability assay as well as the plasma protein binding of 5-nitroimidazole compounds