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Overnight incubation of RNase A MESNA with Cys-PEG-DSPE in a pH 7.4 HEPES buffered saline (HBS) and 50 mM 4-mercaptophenylacetic-acid (MPAA) as a catalyst resulted in a ligation yield of ~50% as estimated from the intensities of the bands of the SDS-PAGE gel

Acknowledgements This work was supported by the National Natural Science Foundation of China [grant number 62376256], Research Funds of Centre for Leading Medicine and Advanced Technologies of IHM [grant number 2023IHM01041], Clinical medical research Transformation Project of Anhui Province [Grant Number 202204295107020020], and the Joint Fund for Medical Artificial Intelligence [Grant number MAI2022Q011]

Consequences of total and subtotal myeloperoxidase deficiency: risk or benefit

Unprocessed western blots Source Data Extended Data Fig