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Once reconstituted, store at 4C and use within 30 days for most peptides
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However, this DOR mutant cannot internalize anymore when arrestin 3 expression is knocked-down suggesting that the non-phosphorylated DOR can internalize but in an arrestin 3-dependent manner
The rats were allowed to acclimate for one week under constant temperature (25 2 C) and a 12-hour light/dark cycle
