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doi: 10.1007/s00705-006-0880-2 85 ZhangK.ShenY.WangT.WangY.XueS.LuanH.et al

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ECM proteins must be coated immediately after polymerization, because NHS-AA ester is quickly hydrolyzed, whereas gels for sulfo-SANPAH experiments can be stored for approximately 3 days prior to sulfo-SANPAH conjugation before the stiffness changes ( In this study, we have developed a novel preparation method for PA gels using NHS-AA ester as a protein crosslinker