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AHK-Cu is a synthetic copper-binding tripeptide extensively used in pre-clinical research to investigate dermal papilla cell activity, hair follicle biology, angiogenic signaling, and extracellular matrix dynamics in skin models

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Some meta-analyses show modest improvements in certain markers depending on population, baseline health, and dose, while others show limited impact

For fluorescence microscopy, cells were serially diluted, added to an agarose pad (prepared by dissolving 0.5% agarose in TAE buffer and spreading it over a glass slide in the form of pad), and imaged on a fluorescence microscope (Leica DM 2500) under 100X objective with GFP filter