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In slow-growing egg-type chicks, carotenoid exposure in ovo and in the food after hatch reduces markers of inflammation in the liver (e.g., change in liver weight during acute phase response) and spleen (e.g., IL-6 mRNA abundance)

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Furthermore, elevated methylation levels of peroxisome proliferator-activated receptor gamma (PPARG) suppress its transcriptional activity, impairing adipocyte differentiation and glucose homeostasis, which contributes to the development of obesity and metabolic syndrome in PCOS patients (51)

Measurements of metabolite-pairs to determine the redox state appear to be most important for studies determining the effects of fasting or DR on the mitochondrial [NAD + ]/[NADH], where measurements of mitochondrial total NAD + /NADH following fasting showed either no change or an oxidizing redox shift, opposite to the reducing redox shift indicated by measurements of the tissue acetoacetate/beta-hydroxybutyrate indicative of the mitochondrial (free) [NAD + ]/[NADH] (Hagopian et al., 2003