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64 ORFs are predicted from genome data and compared against a preset library to predict putative RiPP BGCs, dependent on the presence of known RiPPs motifs, homology to known RiPPs, and class-specific PFAM domains, including leader sequences

ultracentrifuge (Optima XPN-100, Beckman Coulter, USA)

Consequences of this regulation include prolonged insulin receptor activation, activation or inhibition of AKT2, in part mediated by PTEN and PTP1B, as well as regulation of genes involved in cell fate, metabolism, or protein homeostasis through FOXO [43]

Gut microbial-derived phenylacetylglutamine accelerates host cellular senescence