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Mass spectrometry HRPII-PH001D5 protein bands were in-gel digested with AspN (Promega) and eluted peptides, or synthetic peptides incubated with PMV-agarose, were fractionated by reversed-phase liquid chromatography on a nanoflow HPLC system (1200 series, Agilent, USA) using a nanoAcquity C18 150 mm 0.15 mm I.D

Optimization studies employing single-factor design, BBD, and response surface methodology achieved a BSP yield of 43.95% 0.26% under IAE (Qu et al., 2016), representing a 19% increase over conventional hot water extraction

Unfortunately, the existing studies have so far not considered this caveat