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Reduction and alkylation was performed by adding Tris(2-carboxyethyl)phosphine (TCEP) and 2-Chloracetamide (CAA) at the final concentrations of 10 mM and 40 mM, respectively, and incubating them for 5 min at 45 C

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Mar Drugs 20(10):622 Li L, Chen D, Lin X, Luo J, Tan J, Li P (2023) Understanding the role of inflammation in sensorineural hearing loss: current goals and future prospects
