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Description
2-DE separates proteins into multiple spots based on their isoelectric point and molecular weight, whereas Western blotting (WB) detects the total amount of the entire protein
They are frequently investigated together because their potential mechanisms of action are thought to be complementary, creating a synergistic effect that is a popular subject of preclinical studies
Twelve hours later, animals are washed from their plate with M9 into a glass conical tube to a final volume of 600 l
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