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Specifically, the system consisted of a Thermo Q-Exactive in line with an electrospray source and an Ultimate3000 series HPLC consisting of a binary pump, degasser and auto-sampler outfitted with an Xbridge Amide column (Waters) (dimensions: 3.0 100 mm and a 3.5-m particle size)

Mol Pharmacol 93(4):251258 Lu M, Wu B (2016) Structural studies of G protein-coupled receptors

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Proper peptide handling during preparation and storage helps reduce degradation processes such as hydrolysis, oxidation, and peptide aggregation that may affect analytical performance in experimental systems