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In contrast, the bulkier Y37 rAmy in rAmy-bound AMY 2 R prevents equivalent interactions of N35 rAmy with RAMP2, and instead the bulkier Y37 rAmy forms interactions with E101 RAMP2 and F111 RAMP2 stabilising the peptide at the CTR/RAMP ECD interface in the bypass conformation

M.McInnisM

The switch is straightforward since only the vitamin additive changes

For RNaseq, contaminating genomic DNA was removed using RNase-Free DNase (QIAGEN) and samples were cleaned up with the RNeasy Mini Elute Cleanup Kit (QIAGEN)
