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The beads were washed at RT three times in capbinding buffer and boiled with 2X loading dye, followed by separation by SDS-PAGE and immunoblotting

Between 10 to 30 percent of people over 50 years old produce too little stomach acid, leading to decreased absorption of B12 from food

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Additionally, exogenous SA supplementation has helped to develop tolerance to drought, salt, heavy metal toxicity, and temperature extremes (Paul et al., 2024)
