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Description
Methyl-B12 is closely allied with the folic acid biochemical pathway
Tons of veggies
For measurement of metabolite levels in cells, hepatocytes were harvested by treating with 0.25 % trypsin (Gibco #25200), washing with ice-cold PBS to remove trypsin, suspending released hepatocytes in ice-cold PBS, and centrifuging the cell suspension at 1600 g for 10 min at 4 C to obtain the pelleted cells, which were immediately frozen and stored at 80 C for later analysis
2015;10(7):e0131177
