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Cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated FBS, L-glutamine, penicillin, and streptomycin at 37 C in 5% CO 2

A further strategy was to increase the function of the T lymphocytes, by activating two distinct activation pathways

Previously, a double-blind clinical trial reported that the co-ingestion of 120 mg of a Ginkgo biloba extract together with 500 mg metformin did not significantly change the metabolism of metformin in healthy individuals or in individuals with type 2 diabetes mellitus (Kudolo 2006)

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