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Briefly, Vero cells adhered at a density of 110 4 cells/mL in 96 microtiter plates, and ROS levels were measured after 6 hours of treatment with erastin (8 M) supplied with Fer-1 (60 M), fer-1 (60 M), erastin (8 M), and a blank control

The replacement of critical metal ions with harmful heavy metals leads to a decrease in enzyme activity

Nevertheless, the advantage of the FPT over the other existing parametric methods is in the most accurate quantification and time signal extrapolation based on Pad rational polynomials

The samples were treated with tris (2-carboxyethyl) phosphine (10 L, 55 mM in 50 mM TEAB, 30 min, 60 C) followed by treatment with chloroacetamide (10 L, 120 mM in 50 mM TEAB, 30 min, 25 C in the dark)