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The commercial availability of spherical hydroxyapatite has made hydroxyapatite column chromatography an accessible technique [48]

In addition, BCAAs can induce the formation and secretion of disulfide-group-modified high-mobility group box 1 protein (HMGB1) in macrophages through the mitochondrial-nuclear H2O2 signaling pathway, which activate pro-inflammatory macrophages and trigger inflammation via the HMGB1/Toll-like receptor 4 (TLR4)/NF-B pathway, promoting the progression of atherosclerosis (63)

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The high abundant CM10 was speculated to contain monohydroxylation on the 5-ethyl side chain, which was in agreement with previously published data [27, 36]