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GoldBio Nickel Agarose Bead Binding Capacity Nickel Agarose Bead Type Binding Capacity GoldBio Catalog # Highest Density Nickel 80 mg/mL H-390 Nickel NTA Magnetic 75 mg/mL H-351 Nickel NTA HTC 60 mg/mL H-355 Nickel NTA 50 mg/mL H-350 Nickel Agarose Beads (High Density) 25 mg/mL H-320 Nickel HTC 6 mg/mL R-202 For a binding capacity of 80 mg/mL, this means that 80 mg of protein should bind to 1 mL of agarose resin
Ok, now lets consider a scenario in which you already have a candidate interacting protein, and want to determine which part of it interacts with your protein of interest (Figure 6)
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