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Similarly, a study based on swimming exercise for a total of four hours showed an increase in NAD + levels, yet also in this case, no measurements of mitochondrial function were performed 309 , reinforcing the need for more exercise-based investigations to further define how NAD + and mitochondrial homeostasis are perturbed and connected during exercise

While melt shift analysis helps identify proteins with biophysical changes, TPCA is a method that more specifically monitors binary protein interactions and protein complex dynamics (75)

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