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Serious adverse events are rare in anecdotal reports, but anecdotal data cannot substitute for controlled trials
Oxytetracycline (Sigma-Aldrich) was added to the urine collection vessels of the metabolic cages to prevent bacterial deterioration

Diabetologia 38:14251433 Ziegler D, Hanefeld M, Ruhnau KJ, Hasche H, Lobisch M, Schutte K, Kerum G, Malessa R (1999) Treatment of symptomatic diabetic polyneuropathy with the antioxidant alpha-lipoic acid: a 7-month multicenter randomized controlled trial (ALADIN III study)

Following the removal of the secondary antibody, the cells were fixed using an anti-fading fixing medium
