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Quantitative real-time PCR was then performed using SYBR Green master mix (Vazyme, Nanjing, China), with primer sequences detailed in Supplementary Table 1
Afterwards, the membranes were incubated with the correspondent secondary antibody and a chemiluminescent HRP substrate (Merck Millipore
Glutathione supports cellular immunity and reduces oxidative stress
Unlike topical sunscreens that physically block or absorb UV radiation before it penetrates the skin, astaxanthin's primary mechanism involves neutralizing the reactive oxygen species generated by UV exposure after it occurs
