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Total RNA was harvested from monolayer and pellet cultures at different time points using Qiazol (Qiagen, Germantown, MD, United States) and purified with RNeasy Plus Universal Mini Kit (Qiagen, Cat
In addition to acute medicine use (see: Prescribe a NSAID, corticosteroid or colchicine to treat gout flares), encourage rest and elevation of the affected joint, adequate hydration, and some patients may find an ice pack beneficial
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When a patient is lacking or losing response and TDM shows sufficiently high serum trough levels of IFX, switching to another therapy should be considered
