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Disulfiram modulated ROS-MAPK and NFkappaB pathways and targeted breast cancer cells with cancer stem cell-like properties

For the construction of scFv-A36 mutants, a DNA fragment, including the CDR1 and CDR3 regions of the heavy chain of A36, was amplified using PCR and the following degenerate primers: HindIII (underlined) sense linker primer (5-GCA AAGCTT CTGGCTTCNNNNNNNNNNNNNNNNNNNNNNNNTGGGTGAAGCAGAGGCCTGCACAGG-3) and BstEII (double underlined) antisense linker primer (5-GGAGAC GGTGACC GTGGTCCCTTGGCCCCANNNNNNNNNNNNNNNNNNNNNNNNAGCACAGTAATAGACGGCAGTGTCCTCAG-3), in which N is A, C, G, or T (equimolar)

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Hyperactivation of oxidative mitochondrial metabolism in epithelial cancer cells in situ: visualizing the therapeutic effects of metformin in tumor tissue