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The beads were then washed and boiled with 1 SDS loading buffer for SDSPAGE analysis, followed by Coomassie blue staining for liquid chromatography (LC)MS analysis, or the proteins were transferred onto nitrocellulose membrane and incubated with antibodies

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193 Replication stress in GSCs, associated with prolonged transcription of long neural genes, results in increased reliance on DNA damage response pathways, including ATR and checkpoint kinase 1 (Chk1) activation