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The tissue preparation is laborious, as the process requires EPON resin embedding, and is most effective when staining semi-thin (0.5-1 m) plastic-embedded tissue sections

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How is all of this different from steroids and surgery

The mean intensity for collagen 3 had still a significant difference for load levels ( p = 0.013), elastin had a trend ( p = 0.067), while the effect of load on collagen 1 diminished